Figure 7. RasD expression is heterogeneous in growth phase populations.
(A) Dual promoter vectors used to drive constitutive GFP expression and gefE promoter (left) or rasD promoter (right) driven RFP expression. Cells growing in tissue culture plates were photographed with a fluorescence microscope on red channel (a and d), green channel (b and e) and both (c and f). (B) Ax3 cells transformed with rasD promoter vector were fractionated into RFP high and RFP low populations by FACS. These populations were mixed in a 5:95 ratio with unlabelled Ax3 cells. Cell fate choice was traced by constitutive expression of GFP at slug (a and b) and culminant (c and d) stages. White bars show regions of fewer GFP cells. (C) FACS sorted low or high rasD:RFP cells were cultured back in HL-5 medium. The ratio of RFP:GFP cells was scored over time.
Figure 7—figure supplement 1. RasD is expressed in prestalk cells during development.
