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. Author manuscript; available in PMC: 2014 Oct 22.
Published in final edited form as: Biochemistry. 2013 Oct 8;52(42):10.1021/bi401117y. doi: 10.1021/bi401117y

Figure 3.

Figure 3

A Michaelis-Menten plot which shows the dependence of vi/[E] on the concentration of substrate h-FUMP for the deuterium exchange reaction catalyzed by the R235A mutant of ScOMPDC at pD 8.15 (20 mM GlyGly, 20% free base), and I = 0.14 (NaCl). The solid line shows the correlation of the experimental data to eq 2 derived for Scheme 3, using the values for the kinetic parameters reported in Table 1.