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Dependence of the observed second-order rate constant (kcat/Km)obs for the R235A mutant of ScOMPDC-catalyzed decarboxylation of the truncated substrate FEO on the concentration of added phosphite dianion at pH 7.0, 25 °C and I = 0.14 (NaCl). The solid line shows the linear correlation of the experimental data to eq 3, derived for Scheme 4, using the values for the kinetic parameters reported in Table 2.
