Table 1.

Kinetic Parameters for the Decarboxylation and Deuterium Exchange Reactions Catalyzed by Wildtype and R235A ScOMPDC.^a

	kcat or kex (s−1)		Km or Kd (M)		kcat/Km or kex/Kd (M−1 s−1)
	Wildtype	R235A	Wildtype	R235A	Wildtype	R235A
OMP b	15	1.0	1.4 × 10−6	1.1 × 10−3	1.1 × 107	910
Effect of mutation	15-fold decrease		790-fold increase		12000-fold decrease
FOMP c	95	92	8 ×10−6	5.8 × 10−4	1.2 × 107	1.6 × 105
Effect of mutation	No effect		73-fold increase		75-fold decrease
h-FUMP d	0.044	9.3 × 10−6	1.1 × 10−4	5.0 × 10−3	400	1.9 × 10−3
Effect of mutation	4700-fold decrease		45-fold increase		210000-fold decrease

^aThe kinetic parameters k_cat and K_m, and k_ex and K_d, respectively, are used for the ScOMPDC-catalyzed decarboxylation and deuterium exchange reactions.

^bAt pH 7.1 (30 mM MOPS) 25 °C, and I = 0.10 (NaCl). Data for wildtype ScOMPDC is from Ref. 38.

^cAt pH 7.1 (10 mM MOPS) 25 °C, and I = 0.10 (NaCl). Data for wildtype ScOMPDC is from Ref. 39.

^dAt pD 8.15 (20 mM GlyGly, 20% free base), and I = 0.14 (NaCl). Data for wildtype ScOMPDC is from Ref. 13.