Table 2.

Kinetic Parameters from Scheme 4 for the Decarboxylation Reactions of the Substrate Pieces Catalyzed by Wildtype and R235A Mutant ScOMPDC.^a

ScOMPDC	EO + HPO32−		FEO + HPO32−
	(kcat/Km)E (M−1 s−1)	$\frac{{(k_{\text{cat}}/K_{\mathrm{m}})}_{\mathrm{E}•\mathrm{H}\mathrm{P}\mathrm{i}}}{K_{\mathrm{d}}}$ (M−2 s−1)	(kcat/Km)E (M−1 s−1)	$\frac{{(k_{\text{cat}}/K_{\mathrm{m}})}_{\mathrm{E}•\mathrm{H}\mathrm{P}\mathrm{i}}}{K_{\mathrm{d}}}$ (M−2 s−1)
Wildtype b	0.026	1.2 × 104	10	4.8 × 104
R235A	0.026 c	0.63 c	12	250 ± 15

^aThe second-order rate constants reported in this Table are reproducible to better than ± 10%.

^bThe data for the wildtype ScOMPDC catalyzed reactions of EO and FEO are from Ref. 19 and 28, respectively.

^cRef. 19.