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. 2013 Nov 14;170(7):1384–1395. doi: 10.1111/bph.12328

Figure 3.

Figure 3

Effects of chronic pressure overload on cardiac fibrosis in wild-type (WT) and angiotensin II type 1a receptor knockout (AT1aR-KO) mice. (A–D) Left ventricular expression of fibrotic marker genes 4 weeks after transverse aortic constriction (TAC) or sham operation. Relative expression levels of TGF-β1 (A), type 1 collagen (B), fibronectin (C) and CTGF (D) mRNA were normalized to corresponding GAPDH mRNA levels. The mean relative level of each mRNA in sham-operated WT mice was assigned a value of 1.0. Values are means ± SEM (n = 8 each). *P < 0.01 versus sham-operated mice of each genotype. (E) Sirius red-stained sections of left ventricle from the both AT1aR-KO and WT mice processed 4 weeks after TAC or sham operation. Scale bars are 1 mm. (F) The relative fibrotic areas in left ventricles from AT1aR-KO and WT mice 4 weeks after TAC or sham operation. The mean value of the relative fibrotic area in left ventricles from sham-operated WT mice was assigned a value of 1.0. Values are means ± SEM (n = 8 each). *P < 0.01 versus sham-operated mice of each genotype.