Figure 5.

Plasmin-mediated increases in cell number are regulated by the PI3K/Akt and ERK1/2 MAPK signalling pathways. Phosphorylation of (A) Akt- and (B) ERK1/2 following incubation with plasmin (5 mU·mL⁻¹) for 30 min in the presence of LY294002 (LY, 10 μM) or PD98059 (PD, 10 μM). Inserts show representative Western blots. (C) The effect of LY294002 and PD98059 on cell number following incubation with plasmin (5 mU·mL⁻¹) for 48 h. *P < 0.05, **P < 0.01 (n = 4–6).