Figure 6.

EGF-receptor (EGFR) kinase inhibition and EGF receptor knock down attenuates plasminogen-mediated increases in cell number. (A) Cell number and (B) MTT reduction following incubation with plasminogen (Plg, 5 μg·mL⁻¹), plasmin (Plm, 5 mU·mL⁻¹) or EGF (300 pM) with either ilomastat (ilom, 2.5 μM), AG1478 (0.5 μM) or SB431542 (10 μM) for 48 h. (C) Levels of EGF receptor mRNA in cells transfected with EGF receptor siRNA. (D) Cell number in EGF receptor siRNA transfected cells following incubation with plasmin (5 mU·mL⁻¹) for 48 h. (E) Levels of HB-EGF detected in the culture supernatants of cells incubated with plasmin for 48 h. *P < 0.05, **P < 0.01 (n = 4–8).