Figure 3.

Effects of prucalopride on cholinergically mediated contractions evoked by EFS of the human isolated colon circular muscle. Typical traces are shown in the upper panels in the absence (A) and presence (B) of L-NAME 300 μM; the enlarged section of tracing obtained in the absence of L-NAME shows the ability of prucalopride to act mostly by increasing muscle contractions during EFS. The graph below (C) shows the concentration-response curve for prucalopride in the absence of L-NAME, measuring the mean (±SEM) increase in EFS-evoked AUC. EFS (50 V, 0.5 ms bipolar pulse duration, 5 Hz) was given for 10 s, every 1 min (n = 3–6 patients for each data point).