Figure 5.

Langevin dynamics simulation of rotary catalysis by F₁. (a) Simulated time course of θ_B and θ_γ based on the simulation model for P_i release. Red, blue, green, orange, and purple represent the results of θ_B for ϕ = 40, 60, 80, 200, and 500 nm, respectively. Black time courses represent the results of θ_γ. (b) Simulation results of catalytic rate and viscous drag of rotary probes. Red, orange, and blue data points represent the experimental results for P_i release, ATP hydrolysis, and ATP binding of wild-type F₁, respectively, whereas green points represent ATP hydrolysis by the mutant F₁ (α₃β(E190D)₃γ). The pink and light blue lines represent the simulation results for P_i release and ATP binding, respectively, by wild-type F₁; the light green line represents ATP hydrolysis by the mutant F₁. To see this figure in color, go online.