Figure 6.

Inhibition of hepatic PP2A results in inactivation of GS and activation of Phosphorylase. LB1-treatment resulted in increased phosphorylation (Ser641) and inactivation of GS (a) while increasing the ratio of active Phosphorylase a to inactive b (b) in the livers of fat-fed rats. Cantharidin-treatment in primary rat hepatocytes mimicked these effects and resulted in inactivation of GS (c) and activation of Phosphorylase (d). Data are averages of western blot quantifications ±SEM. * P<0.05. Representative western blots are shown.