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. 2013 Nov 7;2013:451349. doi: 10.1155/2013/451349

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Copyright © 2013 S. Pucci et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Confocal microscopy examination of triple stain in a culprit lesion (coronary artery from AMI group). (a) (magnification 2000x) shows a 2D reconstruction triple stain for T cells, CD3 (blue), fractalkine receptor CX3CR1 (green), and IFN-gamma (red); yellow, light blue, and white areas were due to multiple positivity. (b) CD3 immunostaining revealed by coumarin conjugated streptavidin; (c) CX3CR1 immunostaining revealed by FITC conjugated streptavidin; (d) IFN-gamma immunostaining revealed by Texas Red conjugated streptavidin.