Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1979 Aug;76(8):3627–3631. doi: 10.1073/pnas.76.8.3627

Transcriptional regulation of the yeast cytochrome c gene.

R S Zitomer, D L Montgomery, D L Nichols, B D Hall
PMCID: PMC383885  PMID: 226972

Abstract

DNA from the cloned yeast iso-1-cytochrome c, cycl, gene was used in a hybridization assay to measure levels and rates of synthesis of cycl RNA. Derepressed cells synthesized cycl RNA at 6 times the rate of that of glucose-repressed cells. Upon glucose addition to a derepressed culture, the transcription of the cycl gene was repressed within 2.5 min. The half-life of hybridizable cycl RNA was determined to be 12-13.5 min under repressed and derepressed conditions and during repression. The results demonstrate that the expression of the cycl gene is subject to transcriptional regulation.

Full text

PDF
3627

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bach M. L., Lacroute F., Botstein D. Evidence for transcriptional regulation of orotidine-5'-phosphate decarboxylase in yeast by hybridization of mRNA to the yeast structural gene cloned in Escherichia coli. Proc Natl Acad Sci U S A. 1979 Jan;76(1):386–390. doi: 10.1073/pnas.76.1.386. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Darnell J. E., Jelinek W. R., Molloy G. R. Biogenesis of mRNA: genetic regulation in mammalian cells. Science. 1973 Sep 28;181(4106):1215–1221. doi: 10.1126/science.181.4106.1215. [DOI] [PubMed] [Google Scholar]
  3. Elwell L. P., De Graaff J., Seibert D., Falkow S. Plasmid-linked ampicillin resistance in haempohilus influenza type b. Infect Immun. 1975 Aug;12(2):404–410. doi: 10.1128/iai.12.2.404-410.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kennell D., Bicknell I. Decay of messenger ribonucleic acid from the lactose operon of Escherichia coli as a function of growth temperature. J Mol Biol. 1973 Feb 15;74(1):21–31. doi: 10.1016/0022-2836(73)90351-3. [DOI] [PubMed] [Google Scholar]
  5. Montgomery D. L., Hall B. D., Gillam S., Smith M. Identification and isolation of the yeast cytochrome c gene. Cell. 1978 Jul;14(3):673–680. doi: 10.1016/0092-8674(78)90250-7. [DOI] [PubMed] [Google Scholar]
  6. Puga A., Borrás M. T., Tessman E. S., Tessman I. Difference between functional and structural integrity of messenger RNA. Proc Natl Acad Sci U S A. 1973 Jul;70(7):2171–2175. doi: 10.1073/pnas.70.7.2171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Sherman F., Stewart J. W., Jackson M., Gilmore R. A., Parker J. H. Mutants of yeast defective in iso-1-cytochrome c. Genetics. 1974 Jun;77(2):255–284. doi: 10.1093/genetics/77.2.255. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Sherman F., Taber H., Campbell W. Genetic determination of iso-cytochromes c in yeast. J Mol Biol. 1965 Aug;13(1):21–39. doi: 10.1016/s0022-2836(65)80077-8. [DOI] [PubMed] [Google Scholar]
  9. Singh A., Sherman F. Deletions of the iso-1-cytochrome c and adjacent genes of yeast: discovery of the OSM1 gene controlling osmotic sensitivity. Genetics. 1978 Aug;89(4):653–665. doi: 10.1093/genetics/89.4.653. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Smith M., Leung D. W., Gillam S., Astell C. R., Montgomery D. L., Hall B. D. Sequence of the gene for iso-1-cytochrome c in Saccharomyces cerevisiae. Cell. 1979 Apr;16(4):753–761. doi: 10.1016/0092-8674(79)90091-6. [DOI] [PubMed] [Google Scholar]
  11. Zitomer R. S., Hall B. D. Yeast cytochrome c messenger RNA. In vitro translation and specific immunoprecipitation of the CYC1 gene product. J Biol Chem. 1976 Oct 25;251(20):6320–6326. [PubMed] [Google Scholar]
  12. Zitomer R. S., Nichols D. L. Kinetics of glucose repression of yeast cytochrome c. J Bacteriol. 1978 Jul;135(1):39–44. doi: 10.1128/jb.135.1.39-44.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES