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An immunoblot of claudin-4 for neosquamous epithelium (NSE) and for native esophageal upper squamous epithelium (USE). Ten micrograms of protein were loaded in each lane and the actin signal shown to document equal protein loading. Molecular weight standards are displayed on the left in kilodaltons (kDa). Prestained SDS-PAGE standard (wide range; Bio-Rad) was used (red bands). Claudin-4 is shown to be more strongly expressed in USE than NSE.
