Table 2.
The average number of plasmids per nanoparticle for the seven nanoparticle formulations as determined by nanoparticle tracking analysis (NTA).
| Polymer | wt/wt | Mode plasmid/particle | Average plasmid/particle | Standard Error | Number of Tracks | Average theoretical max plasmid/particle | Average kbp/particle | Average theoretical max kbp/particle |
|---|---|---|---|---|---|---|---|---|
| B4S4E7 | 40 | 57 | 120 | 20 | >500 | 127 | 564 | 597 |
| B4S4E7 | 60 | 63 | 110 | 10 | >500 | 195 | 517 | 917 |
| B4S5E7 | 60 | 29 | 90 | 30 | >500 | 119 | 423 | 559 |
| B5S3E7 | 60 | 23 | 45 | 9 | >500 | 110 | 212 | 517 |
| B5S3E7 | 100 | 17 | 30 | 2 | >500 | 80 | 141 | 376 |
| B5S5E1 | 100 | 23 | 35 | 2 | >500 | 86 | 165 | 404 |
| PEI 25kDa | 2 | 49 | 90 | 10 | >500 | 267 | 423 | 1255 |