Fig. 3.
Liver H & E staining and lipid content in mice. At the end of the study, liver samples were collected and fixed in 10% buffered formaldehyde, and then embedded in paraffin. Slides of liver samples were stained with H&E method. (A) A large amount of lipids accumulated in the livers in the HFD and SANT treated mice, whereas SCO decreased lipids in the liver of the mice. (B and C) Hepatic triglyceride and cholesterol content, respectively. (D) The correlation between triglyceride and cholesterol in the liver tissues of all animals. Circle stands for HFD, delta is SANT treated animals, and diamond is the SCO treated mice. SCO significantly reduced lipid accumulation in the liver when compared with SANT and HFD groups. Data are mean ± SEM (n = 5/group). * P < 0.05, SCO vs. HFD, ## P < 0.01, SCO vs. SANT.
