. Author manuscript; available in PMC: 2013 Nov 24.

Published in final edited form as: Biol Blood Marrow Transplant. 2010 Feb 1;16(5):10.1016/j.bbmt.2009.12.007. doi: 10.1016/j.bbmt.2009.12.007

Table 3.

Donor T cell expansion and activation in the bone marrow and liver 3 days after BMT

	Bone Marrow (cells × 10⁵)		Liver (cells × 10⁵)
	CD3⁺CFSE⁺	CD3⁺CFSE⁺CD69⁺	CD3⁺CFSE⁺	CD3⁺CFSE⁺CD69⁺
Syn	0.39 ± 0.17	0.26 ± 0.07	0.14 ± 0.15	0.10 ± 0.22
LN/PP/Sp+/+	0.07 ± 0.00	0.04 ± 0.00	0.43 ± 0.08	0.27 ± 0.07
LN/PP/Sp−/−	0.87 ± 0.00^#	0.72 ± 0.00^#	0.75 ± 0.01^*	0.29 ± 0.08

BMT indicates bone marrow transplantation. T cells (10 × 10⁶) from B10.BR donors were stained with vibrant CFDA SE and infused into lethally irradiated, LN/PP/Sp−/− or LN/PP/Sp+/+ recipients as described in Figure 5. Three days after transfer, single cell suspensions were prepared from the bone marrow cavity (A) or liver tissue (B) and cells from mice in each group were pooled (BM) or processed individually (liver) and subsequently counted, labeled, and analyzed by flow cytometry. Increased numbers of activated and dividing donor T cells are present in the BM of LN/PP/SP−/− mice compared to allogeneic, LN/PP/Sp+/+ littermate controls. Data are presented as mean ± SEM, and represent 1 of 3 similar experiments, n = 4 mice per group per experiment.

P < .05,

P =.06.