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. Author manuscript; available in PMC: 2013 Nov 24.
Published in final edited form as: Nat Immunol. 2012 Dec 23;14(2):10.1038/ni.2509. doi: 10.1038/ni.2509

Figure 3.

Figure 3

Validation of Abcf1 as a regulator of the ISD-sensing response. (a) ELISA of CXCL10 in MEFs treated with control siRNA (siCtrl) or 14 different siRNAs targeting Abcf1 and stimulated with DNA, plotted against qRT-PCR analysis of Abcf1 mRNA expression in corresponding siRNA-treated MEFs. Inset, immunoblot analysis showing knockdown efficiency of siCtrl or siRNAs si-0, si-1 and si-2 targeting mRNA encoding ABCF1 in MEFs; β-actin served as a loading control. (b) ELISA of CXCL10 in MEFs stably expressing doxycycline (Doxy)-inducible HA-tagged Renilla luciferase (Renilla-HA) or Doxy-inducible Abcf1-rescue cDNA treated with siRNA to Abcf1 (si-1) and doxycycline and stimulated with DNA. Bottom, immunoblot analysis showing knockdown efficiency of no siRNA, siCtrl or si-1 in MEFs stably expressing HA-tagged Abcf1 (Abcf1-HA) or HA-tagged siAbcf1(si-1)-knockdown-resistant Abcf1 (Abcf1(rescue)-HA). Data are from one experiment representative of three independent experiments (mean and s.d. in b). *P < 0.05, **P < 0.001 (Student’s t-test).