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. Author manuscript; available in PMC: 2013 Nov 24.
Published in final edited form as: Nat Immunol. 2012 Dec 23;14(2):10.1038/ni.2509. doi: 10.1038/ni.2509

Figure 4.

Figure 4

Small molecule inhibitors modulate the ISD-sensing response. (a) ELISA of CXCL10 in human MoDCs treated with small-molecule inhibitors (500 nM celastrol, 75 nM 17-DMAG or 500 nM BX795) or vehicle control and stimulated with 0.3 μg/ml DNA (HIV gag-100) for 24 h or left unstimulated (–). (b) Immunoblot analysis of TBK1, IRF3, p-IRF3 or CDC37 in MEFs treated with control siRNA (siCtrl) or siRNA targeting Cdc37 (siCdc37) and stimulated for 4 h with DNA. (c) ELISA of CXCL10 in Ptpn1−/− MEFs and Ptpn1−/− MEFs rescued with Ptpn1 cDNA (Ptpn1−/− (rescue)) stimulated with DNA. (d) ELISA of CXCL10 in human MoDCs treated with 7.5 uM PTPN1 inhibitor, 1 nM okadaic acid or vehicle control and stimulated with 0.3 μg/ml DNA (HIV gag-100) or left unstimulated (–). Data are from one experiment representative of three independent experiments (mean and s.d. in a,c,d). *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t-test).