Fig. 5. PTHrP phosphorylated [Y⁴¹⁸] Src family kinases in CD11b⁺Gr1⁺ cells.

(A) Male athymic mice (n=3 per group) were stimulated with a single administration of PTHrP (1–34) or saline control, 8 hours prior to sacrifice and flow cytometric analyses of phospho-[Y⁴¹⁸] Src family kinase expression levels in CD11b⁺Gr1⁺ bone marrow cells. Representative histograms from the control group (shaded) and the PTHrP-stimulated group (open) were overlapped to show the intensity of phospho-[Y⁴¹⁸] Src expression. CD11b⁺Gr1⁺ cells expressing high levels of phospho-[Y⁴¹⁸] Src family kinases (indicated by a bracket ‘M’) were quantified and plotted. Data are mean±SEM. P<0.01, Student’s t-test.

(B) CD11b/Gr1 double positive cells were sorted from the femoral bone marrow of male athymic mice, followed by treatment with saline, IL-6, VEGF-A, CCL-2, RANKL or PTHrP (all 100ng/ml for 0.5×10⁶ cells) for 1 hour at 37°C (n=3/group). Representative histograms (open) were overlapped onto un-stimulated controls (shaded) to show the intensity of staining.