Table 3.

Gene expression analysis in the striatum in YAC128 mice.

Gene symbol	Gene name	Striatum (3 months)		Striatum (6 months)
		Student's t-test	Relative quantification	Relative quantification
Hap1	huntingtin-associated protein 1	*	3.1	1.8
Calb2	calbindin 2 (calretinin)	ns	2.9	2.1
Aph1b	anterior pharynx defective 1b homolog (C. elegans)	***	2.9	1.9
Psenen	presenilin enhancer 2 homolog (C. elegans)	*	2.3	1.5
Aph1a	anterior pharynx defective 1a homolog (C. elegans)	**	2.3	1.9
Psen2	presenilin 2	*	2.0	1.6
Cib1	calcium and integrin binding 1 (calmyrin 1)	**	2.0	1.3
CacyBP/SIP	calcyclin binding protein	*	1.8	1.6
Aph1c	anterior pharynx defective 1c homolog (C. elegans)	*	1.7	1.9
Calr	calreticulin	*	1.5	1.6
Cib2	calcium and integrin binding family member 2	**	1.5	1.8
Bace1	beta-site APP cleaving enzyme 1	*	0.7	0.8
Rgs4	regulator of G-protein signaling 4	*	0.8	0.9

The striatum from 3- and 6-month-old YAC128 mice was compared to age-matched control mice using custom-made TaqMan low-density PCR arrays. Only statistically significant gene expression changes are shown:

^*p < 0.05,

^**p < 0.005,

^***P < 0.0005, with the exception of Calb2. Cut-offs of relative quantification (RQ) > 1.3 for upregulated genes and RQ < 0.75 for downregulated genes were applied as criteria for differential expression in transgenic mice, with the exception of Rgs4, which did not meet the additional criterion RQ < 0.75. The results for control mice were normalized to a value of 1. The gene expression results were normalized to GAPDH (n = 3 independent biological samples for 3-month-old YAC128 and age-matched control mice). The final concentration of cDNA for the RT-qPCR array was 1.6 μg. This amount contained the combination of equal amounts of cDNA obtained either from the striatum of three 6-month-old transgenic HD mice or from three age-matched control mice.