Figure 1.

BH scale analysis of P2X C-terminal regions. The analysis of all rat P2X C-terminal regions using the BH scoring method (Brzeska et al., 2010) predicts strong PIP_n affinity for the reported PIP_n binding region in the subunits experimentally shown to directly bind PIP_n. A BH score above 0.6 was demonstrated to accurately identify unstructured lipid-binding sites in proteins. The input sequence consisted of 60 amino acids of the C-terminal region, including the last 13 amino acids of the second transmembrane domain (starting at residue Gly324 in P2X1 numbering). The analysis was performed with a window size of eight amino acids.