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. Author manuscript; available in PMC: 2014 Oct 21.
Published in final edited form as: Chem Res Toxicol. 2013 Oct 8;26(10):10.1021/tx400293s. doi: 10.1021/tx400293s

Figure 1. Formation of ICLs during reduction of Cr(VI) with GSH.

Figure 1

Reaction mixtures contained 10 mM GSH, 25 mM MOPS (pH 7.0), 2 μg DNA and indicated concentrations of potassium chromate. All reduction reactions were incubated for 3 hr at 37°C. Data are means+SD (n=3-4). Where not seen, error bars are smaller than data symbols. (A) Reduction of 100 μM Cr(VI) by GSH. (B) Formation of Cr-DNA adducts in Cr(VI)-GSH reactions. (C) Representative radiogram demonstrating the presence of dsDNA in Cr(VI)-treated samples after denaturation with 200 mM NaOH. (D) Dose-dependence of ICL formation by Cr(VI). (E) Yield of ICLs as a percentage of total Cr-DNA adducts.