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. Author manuscript; available in PMC: 2014 Oct 21.
Published in final edited form as: Chem Res Toxicol. 2013 Oct 8;26(10):10.1021/tx400293s. doi: 10.1021/tx400293s

Figure 5. Importance of FANCD2 for recovery of cell cycle after exposure to mitomycin C but not Cr(VI).

Figure 5

(A) A flow chart describing an experimental approach for testing the ability of cells to recover from replication arrest and progress into mitosis. (B) A severe defect in the ability of FANCD2-null cells to recover from mitomycin C (MMC)-induced replication arrest. Cells were trapped in mitosis by the addition of 0.1 μg/mL nocodazole for 18 hr after removal of MMC. (C) A normal progression of FANCD2-null cells into mitosis after Cr(VI) exposure. Cells were trapped in mitosis by the addition of 0.1 μg/mL nocodazole for 18 hr after Cr(VI) exposure.