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. 2013 Nov 25;3:3325. doi: 10.1038/srep03325

Figure 3. Suppressive effect of novel RNAi agents for RPN2 in A549-luc-C8 cells.

Figure 3

(a) Immunohistochemical staining for RPN2 proteins in representative tumors of A549-luc-C8 xenograft lung cancer models. The scale bars indicate 100 μm. (b) Phase-contrast micrographs of A549-luc-C8 cells 96 h after transfection with RPN2 siRNA, RPN2 PnkRNA, RPN2 nkRNA or control siRNA using DharmaFECT 1 reagent. The scale bars indicate 10 μm. (c) Cell proliferation was measured 96 h after transfection with each of the RNAi therapeutic agents. Inhibition of cell growth was observed on A549-luc-C8 cells treated with RPN2 siRNA, PnkRNA, nkRNA, or the control siRNA. Statistical analysis was performed by the Bonferroni multiple-comparison test. The data represent the means ± SD (n = 3). ***, P < 0.001 versus control siRNA group. **, P < 0.01 versus control siRNA group. *, P < 0.05 versus control siRNA group. (d) The inhibition of the targeted mRNA levels is shown. Human RPN2 expression levels were normalised to beta-actin levels. Statistical analysis was performed by the Bonferroni multiple-comparison test. Data represent the means ± SD (n = 3). ***, P < 0.001 versus control siRNA group.