Table III.
Summary of Kinetic Parameters
| Enzyme | Specific activity (nmol/min/mg) | kcat (s−1) | Km(μM ATP) | kcat/Km (M−1s−1) |
|---|---|---|---|---|
| Adenylate kinase avtivity (ATP + AMP↔2ADP) | ||||
| hCINAPWT | 18 ± 0.9 | 6.3 × 10−3 | 45 ± 5 | 140 |
| hCINAPH79G | 11 ± 0.16 (nH = 1.99a) | 3.7 × 10−3 | 93 ± 8a | 39 |
| ATPase activity (ATP→ADP + Pi) | ||||
| hCINAPWT | 1.37 ± 0.08 (nH = 1.21a) | 4.8 × 10−4 | 332 ± 8a | 1.45 |
| hCINAPH79G | 0.74 ± 0.04 (nH = 2.10a) | 2.5 × 10−4 | 721 ± 1a | 0.34 |
a
Best-fit curve obtained by fitting the data to the Hill equation. Km and nH were calculated by linear regression of the logarithmically transformed data (Hill plots).