A) Fly ovaries contain strings of ovarioles (arrow). B) Two ovarioles stained with DAPI to label DNA (cyan) and anti-Discs large (Dlg, red) to label membranes. Each ovariole contains progressively developing egg chambers, which mature as they are pushed towards the posterior of the ovary. G=Germarium, NC=Nurse cell, FC=Follicle cell, O=oocyte. C) Schematic showing loss of polar cells (PC, yellow cytoplasm) between stage 3 (left) and stage 5 (right). D) Schematic showing stretch follicle cells (SFC) extending over the nurse cells in a stage 10 egg chamber.