Figure 3. Overview of developmental programmed cell death during late oogenesis.

Egg chambers from well-fed flies stained with DAPI (cyan) to mark the DNA and anti-Discs large (Dlg, red) to mark the cell membranes. A–D) WT egg chambers. A) Stage 11 egg chamber has several NCs that still contain cytoplasm. B) Stage 12 egg chamber has completed dumping and retains NC nuclei. C) Stage 13 egg chamber has begun to form dorsal appendages and has a few NC nuclei remaining. D) Stage 14 egg chamber has fully formed dorsal appendages and no longer contains any NCs. E) Overexpression of DIAP1 in the NCs leads to a weak persisting nuclei phenotype where stage 14 egg chambers still contain a few NC nuclei (arrow). F) deep orange transheterozygous (dor⁸/dor⁴) stage 14 egg chamber has a strong persisting nuclei phenotype with many NC nuclei (arrow).