Fig. 1.

CG5155/Gudu expression pattern and knockdown. (A) Schematic representation of CG5155/Gudu, which encodes a protein with 669 amino acids. The blue boxes indicate 10 predicted ARM repeats based on sequence similarity. The four RNAi lines targeting 3 different regions of the gene are illustrated: RNAi lines 1 and 2 are independent transgenic lines of the same construct with insertions located on chromosome 2 and 3, respectively. (B) By qPCR, male flies express ~10 times more CG5155/Gudu mRNA than the female flies. (C) The CG5155/Gudu mRNA is highly enriched in fly testis. Total mRNA was extracted from the whole male flies, dissected testes, and the carcasses excluding the testes. The CG5155/Gudu mRNA levels were measured using qPCR. A greater than 95% of CG5155/Gudu transcript in male flies is present in testes. The relative mRNA expression of CG5155/Gudu based on qPCR of RNA isolated from (D) whole male and (E) female flies. All 4 RNAi lines knocked down the expression of Gudu to ~15% or lower of the level in wild type males and to ~25% or lower of the level in wild type females. Note that the overall levels in female flies were ~10 times lower than that in male flies as shown in Fig. 1B, although the levels were set to 1 in both control flies in 1D and 1E.