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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Gene. 2013 Sep 17;531(2):10.1016/j.gene.2013.08.080. doi: 10.1016/j.gene.2013.08.080

Fig. 4.

Fig. 4

Knockdown of Gudu disrupts the individualization complex (IC). Rhodamineconjugated phalloidin stained actin, the major component of ICs. DAPI was used to stain the nuclear DNA. GFP tagged Don Juan protein (dj-GFP) illuminated spermatids. Wild type testes show highly organized ICs as a result of coordinated movement of actin cones. All 64 actin cones in each bundle are tightly located tightly together (A’, arrow head). Knockdown of Gudu led to diffused and disorganized ICs (B’ and C’, arrow head). The organized ICs were missing or only very few left in the RNAi lines. Scale bar: 40 μm. Green is dj-GFP, red is phalloidin, and blue is DAPI.