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. Author manuscript; available in PMC: 2013 Nov 25.
Published in final edited form as: Cell. 2012 Nov 21;151(5):10.1016/j.cell.2012.10.039. doi: 10.1016/j.cell.2012.10.039

Figure 1. Phosphorylated Ribosome Profiling.

Figure 1

(A) Neurotransmitters and neuromodulators activate a core set of signaling pathways in neurons. Rps6 is a common target of these pathways and is phos-phorylated on five C-terminal residues.

(B) Asparse subpopulationofneuronsis activated in response a stimulus (red cells). Activated neurons show enhanced pS6, and thus, capture of phosphorylated ribosomes enriches for the mRNA expressed in the activated cells. Quantifying the enrichment (IP/input) of a large panel of cell-type-specific marker genes reveals the genes uniquely expressed in the neurons that were activated.