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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Anal Biochem. 2013 Aug 21;443(1):10.1016/j.ab.2013.08.013. doi: 10.1016/j.ab.2013.08.013

Figure 3.

Figure 3

Specificity of SC-TR assay to TR. A) TR activity measured using the SC-TR assay in which TR1 protein expression has been reduced using si-RNA or increased with a TR1 mammalian expression vector. B-D) SC-TR assay with individual component controls, Protein only (B), master mix only (C), and NADPH only (D). E) Western blot of C10 cells transiently transfected with si-RNA against TR1 (si-TR1) or non-specific scrambled si-RNA (scram). (F)C10 cells transfected with pcDNA 3.1 control vector or increasing concentrations of TR1 expression plasmid. Actin is used as a loading control for E and F.