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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Anal Biochem. 2013 Aug 21;443(1):10.1016/j.ab.2013.08.013. doi: 10.1016/j.ab.2013.08.013

Figure 5.

Figure 5

TR expression and activity in human malignant mesothelioma cell and human mesothelial lysates. A) TR1 and Trx1 protein expression in LP9 mesothelial cells, HM cells, and H2373 malignant mesothelioma cells. Actin is used as a loading control. B) Densitometry of immunoblot signals normalized to actin expression (* p<0.05**p<0.01, ***p<0.001, Student's t-test) C) TR activity measured using the SC-TR assay in 30 μg of protein from indicated cell lysates. The TR activity in LP9 mesothelial cells is 0.51 nmole/min or 17.U/mg. The TR activity in HM cells is 1.1 nmol/min or 35.6 U/mg. The TR activity in H2373 malignant mesothelioma cells is 1.14 nmol/min or 38 U/mg.