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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Anal Biochem. 2013 Aug 21;443(1):10.1016/j.ab.2013.08.013. doi: 10.1016/j.ab.2013.08.013

Table 1.

Volumes of reaction components for selenocystine assay.

Sample Reaction components
Experimental Protein Control NADPH Control SC Control
1 25.5 L protein 25.5 L protein 25.5 L protein 25.5 L protein
34.5 L lysis buffer 74.5 L lysis buffer 54.5 L lysis buffer 34.5 L lysis buffer
40 L master mix 20 L NADPH 40 l SC solution
2 21.3 L protein 21.3 L protein 21.3 L protein 21.3 L protein
38.7 L lysis buffer 78.7 L lysis buffer 58.7 L lysis buffer 38.7 L lysis buffer
40 L master mix 20 L NADPH 40 L SC solution
3 31 L protein 31 L protein 31 L protein 31 L protein
29 L lysis buffer 69 L lysis buffer 49 L lysis buffer 29 L lysis buffer
40 L master mix 20 L NADPH 40 L SC solution