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Alloreactive IFNγ ELISPOT assays performed without SOP. A. Representative replicate wells of IFNγ ELISPOT assays performed using aliquots of the same PBMC responders and B cell stimulators at 2 different sites according to site specific laboratory protocols. B. Quantified ELISPOT results of 4 different responder stimulator pairs as performed at 2 different laboratories.
