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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Am J Transplant. 2013 May 24;13(7):10.1111/ajt.12286. doi: 10.1111/ajt.12286

Figure 2.

Figure 2

Alloreactive IFNγ ELISPOT assay quality determined by multiple technical factors. A. 2 sets of IFNγ ELISPOT assays were compared using 2 different responder stimulator pairs at each of the 2 sites. Results represent means + SD of quadruplicate wells. *p<0.05. B. Representative assay wells of one responder stimulator pair without DNAse added during cell thawing showing typical artifacts (top) or with the addition of DNAse (bottom) C. Captured images of wells from the same plate allowed to dry without removal of the plastic gasket with the wells still slightly wet (top) and after removal of plastic gasket when the wells are fully dry (bottom). D. Representative replicate assay wells performed at 2 sites using the same responder stimulator pairs following the newly devised SOP.