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Variables Addressed Within ELISPOT SOP
| Cell Thawing | Timing of frozen cell pellet transfer into thawing media |
| Inclusion of DNAse in thaw media | |
| Cell Plating | Cell concentrations per well |
| Size of pipette tip orifice | |
| Assay Procedures | Incubation times. |
| Specified number of washes and buffers | |
| Requirement of full drying of plate prior to analysis | |
| Reagents | Antibody: Commercial source, clone and lot numbers |
| Source of developing reagent | |
| Source/recipes of buffers and wash solutions |
