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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Breast Cancer Res Treat. 2013 Sep 24;141(3):10.1007/s10549-013-2703-y. doi: 10.1007/s10549-013-2703-y

Fig. 6. Effect of EPA and DHA on breast cancer cell load and associated osteolysis after intra-tibial injection of breast cancer cell.

Fig. 6

The athymic NCr-nu/nu mice were fed a diet containing CO or EPA-FO or DHA-FO for 4 weeks prior to the intra-tibial injection of the MDA-MB-231 cells. 10 μl PBS containing 1 × 106 cells was injected intra-tibially. The mice were maintained in their respective diets for 4 weeks post injection. After x-ray, mice were sacrificed and bones were collected and fixed in formalin. (B) Osteolytic lesions are shown in x-ray. After decalcification, the bone sections were stained for H&E to determine the tumor burden in bone. Histomorphometry of tumor burden (B) and osteolytic lesions area (D) is shown in bar diagrams. n=5 mice per group. Each value represents the mean ± SEM. Value with different superscripts are significantly different at P<0.05 by Newman Keuls one way ANOVA with multiple comparison test.