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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Nat Neurosci. 2013 Oct 6;16(11):1598–1607. doi: 10.1038/nn.3538

Figure 2. A persistent subpopulation of GFP expressing interneurons co-express somatostatin in CA1 stratum oriens of Htr3a-GFP mice.

Figure 2

(a, b) Representative image and quantification of the distribution of Htr3a-GFP-reported interneurons in the varying lamina of the CA1 subfield (O-A- stratum oriens/alveus; St.P – stratum pyramidale; St.R – stratum radiatum; St.LM – stratum lacanosum moleculare; n = 4 mice and a total of 8 hippocampal sections per mice were examined for counting; total cells counted for O-A, St.P, St.R and St.LM were 1009, 638, 537 and 1420, respectively. (c) Percentage of O-A (black) and St.LM (gray) Htr3a-GFP–reported interneurons that express the various immunocytochemical markers as indicated (n = 3 – 4 mice and a total of 25 – 47 hippocampal sections were examined per marker; number of cells counted for O-A and St.LM were 1621,2062 (CCK); 658,1015 (Calr); 1121,1875 (VIP); 974,1645 (Reelin); 944,1751 (Parv); 744,1335 (SOM), respectively. (d, e) Representative images and quantification illustrating the co-localization between Htr3a-GFP driven GFP and somatostatin in CA1 O-A subfield across a range of postnatal developmental time points (n = 3 – 4 mice and a total of 9 – 11 hippocampal sections were examined per developmental time point; number of cells counted for P5–10, P20, P30 and P60 were 2059, 933, 336, 571 and 476, respectively.). (f) Representative image demonstrating co-localization of somatostatin and GFP-expressing CA1 O-A interneuron (arrow) in the Mash1Cre:RCE reported mouse line following tamoxifen adminstration at E12.5. (g, h) Percentage co-localization between somatostatin and GFP in CA1 O-A interneurons of the Mash1Cre:RCE reported mouse line following tamoxifen administration at varying embryonic developmental stages (n = 3 mice; total number of cells counted at E12.5, E14.5, E16.5 for (g) were 312, 587, 509 and for (h) were 59, 82, 48, respectively). In all cases immunocytochemistry was performed at P30. Box-and-whisker plots are constructed as follows: circle and line within box denotes mean and median, respectively; upper and lower limits of box and capped lines indicate SEM and min/max data points, respectively.