Table 1.
Characteristics of FRAP on published Dictyostelium proteins
| Protein | Half-time (s) | Diffusion coefficient (µm2/s) |
Immobile fraction (%) |
Reference |
|---|---|---|---|---|
| GFP (cytoplasm) | – | 87 ± 2 | 0.0 ± 0.3 | (2) |
| GFP (cytoplasm) + Latrunculin | – | 42 ± 2 | 3.2 ± 0.3 | |
| GFP (nucleus) | – | 22 ± 2 | 4.0 ± 0.3 | |
| Myosin VII (cytoplasm) | 1.0 ± 0.4 | – | ≈50a | (6) |
| Mysoin VII (membrane) | 2.8 ± 1.7 | – | ≈80a | |
| Dynacortin | 0.45 | – | 26 | (10) |
| Fimbrin | 0.26 | – | 33 | |
| Cortexillin I | 3.3 | – | 32 | |
| Myosin II (cortical ring during interphase) | 7.28 ± 1.95 | – | – | (5) |
| Myosin II (contractile ring) | 7.01 ± 2.62 | – | – | |
| MHCK B (contractile ring) | 1.72 ± 0.3 | – | – | (3) |
| MHCK C (contractile ring) | 2.32 ± 0.25 | – | – | |
| Actin (mitosis-specific dynamic actin structures) | 2.15 ± 0.89 | – | – | (7) |
| Coronin | 4.8 ± 1.3 | 2.2 ± 0.2 | – | (1) |
| cAR1 | 18 ± 0.41b | – | ≈60b | Unpublishedb |
| PTEN (membrane) | 2.3 ± 1.6 | – | 23 | Reported here |
“–”Values not reported
a
Average interpretations based on graphs reported
b
Unpublished data from the Janetopoulos lab