. Author manuscript; available in PMC: 2013 Nov 25.

Published in final edited form as: J Neuroophthalmol. 2012 Sep;32(3):10.1097/WNO.0b013e3182589589. doi: 10.1097/WNO.0b013e3182589589

TABLE 2.

Primary antibodies used for immunofluorescence staining (13,17-25).

Antibody	Dilution	Source
Anti-mouse PD-1 (clone 29F.1A12, rat IgG2α) (17)	1:200	Gift of Gordon Freeman, MD, PhD (Boston, MA)
Anti-PD-L1/CD274 (rabbit polyclonal) (17)	1:750	Lifespan Biosciences, #LS-B480
Anti-mouse Brn3a (clone 5A 3.2, mouse IgG1) (18)	1:200	Millipore/Chemicon
Anti-mouse AP2a (clone 3B5, mouse IgG2β) (19)	1:20	Developmental Studies
Hybridoma Bank
Anti-Calbindin D28K (clone CB-955 (formerly CL-300), mouse IgG1) (20)	1:1000	Sigma-Aldrich
Anti-PKCa (clone H-7, mouse IgG1) (21,22)	1:4000	Santa Cruz Biotechnology
Anti-G protein Goa (clone 2A, mouse IgG1) (23)	1:1000	Millipore/Chemicon
Anti-glutamine synthetase (clone GS-6, mouse IgG2α) (24,25)	1:1000	Millipore/Chemicon
Species- or isotype-specific Alexa Fluor 488 or 594	1:1000	Invitrogen
Anti-mouse PD-L2 (clone TY25, rat IgG2α) (13,17)	—	eBioscience
Anti-mouse PD-L2 (clone 19G.4H3, rat IgG2β)	—	Gift of Gordon Freeman, MD, PhD (Boston, MA)
Anti-human PD-L2 (clone 24F.7G12, mouse IgG2α)	—	Gift of Gordon Freeman, MD, PhD (Boston, MA)