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. Author manuscript; available in PMC: 2013 Nov 25.
Published in final edited form as: Biotechnol Bioeng. 2013 Apr 22;110(10):10.1002/bit.24923. doi: 10.1002/bit.24923

Figure 4.

Figure 4

Quantitative analysis of calcium elevation: (a) cell segmentation. A threshold image of the average (left) of stacked images was formed (middle) with the Otsu’s method, and then the individual cells were segmented (right-panel). (b) Analysis of cytoplasmic Ca2+ elevation. Fluorescence temporal changes were obtained from the segmented stacked images (left), and then the cells exhibiting Ca2+ elevation (middle) were found. Finally, the mean of normalized maximum calcium elevation was multiplied by cell responding ratio to give the cell response index, CRI.