Figure 7. NIM811 increases surface area of cultured BHK cells.
(A) Micrograph of BHK cell during patch clamp. 25× LWD lens. Cell diameters were calculated as the average of a horizontal and vertical line transecting each cell, as indicated. The spherical area of each cell was calculated, and the ratio of the electrically determined area to the spherical area was calculated assuming 1pF to be 100 μm2. (B) First three bar graphs give results for BHK cells removed from dishes and incubated at 37°C for 1 or 3 hr without (CTR) and with NIM811 (2 μM). Results for control cells were not significantly different and were pooled. The fourth and fifth bar graphs give results for control cells (CTR) and cells grown with NIM811 (2 μM) for 30 hr.