Figure 3.

mRNA expression measured by RT-PCR of α1, α2, and α3 isoform of Na,K-ATPase in astrocytes isolated by FACS from cerebral hemispheres in vivo from adult mice [FVB/NTg(GFAP-GFP)14Mes/J]. A representative experiment showing mRNAs for α1, α2, and α3 isoform and for TBP, as a house-keeping gene. The sizes of the PCR products of α1 is 920 bp, α2 350 bp, α3 329 bp, and TBP 236 bp, n = 3. From (43).