Table 2.

Drug effects on gene expression and editing in astrocytes are identical in freshly isolated cells from treated animals and primary cultures of astrocytes, but the expression is often not recognized by the microarray analyses, indicatest as + or −.

Protein	Gene	Drug	FACS	Culture	Microarray
					(4)	(6)	(10)	[4*]
5-HT2B receptor expression	Htr2b	Fluox	Up	Up	−	−	−
5-HT2B editing	Htr2b	Fluox	Up	Up
5-HT2c receptor expression	Htr2c	Fluox	Unchanged	Unchanged	−	−	−
cPLA2a	Pla2g4a	Fluox	Up	Up	−	−	−	+
sPLA2	Pla2g2a	Fluox	Unaltered	Unaltered	−	−	−
ADAR2	Adarb1	Fluox	Up	Up	−	−	−	−
GluK2 expression	Grik2	Fluox	Up	Up	+	+	−	−
GluK2 editing	Grik2	Fluox	Up	Up
GluK4 expression	Grik4	Fluox	Unchanged	Unchanged	−	−	−	−
cfos expression	cFos	Fluox	Up	Up	−	+	−	+
fosB expression	Fosb	Fluox	Up	Up	−	+	−	+
NBCe1	Slc4a4	Cbz	Up	Up	+	+	+	+
GluK2	Grik2	Cbz	Down	Down
cPLA2	Pla2g4a	Cbz	Up	Up

The Table shows all experiments in which we have compared drug [fluoxetine (Fluox) or carbamazepine (Cbz)] effects in cultured astrocytes and in astrocytes obtained by FACS. Complete agreement was found between these two preparations, but the correlation was poor with expression of the same genes obtained using microarray analysis and with the cultures, different from ours, studied by Cahoy et al. using microanalysis (4*). The FACS and culture data are from (47) and (48). Microarray data show only gene expression and are from Lovatt et al. [4], Cahoy et al. [6] and Doyle et al. [10] Cahoy et al. (6) also presented data for cultures different from ours, shown under [4]. As in Table 1, expression is shown as either present (+) or absent (−) based on the number values in the microarray analysis itself [2 and 4] or fold-enrichment and significance [1] and [3] shown by Lovatt and Nedergaard (9).