Fig. 3.

Cells in 4× images on microfluidic filters. Enumeration is often required for 4× images, more than 3000 cells per image, with over 1000 cells that fluoresce for both CD3 and CD4. (A,B) correspond to CD3⁺ and CD4⁺ stained cells, respectively. (C) is a merged image of A and B. (D,E,F) are counted versions of A, B, and C. (G,H,I) are the magnified portions of D, E, and F. Counting these images automatically takes 3–5 seconds. Manual counting of these highly populated images take 5 hours. Hence, in this case the automated method provides over 3000 times faster counting. Scale bar corresponds to 200 microns.