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. Author manuscript; available in PMC: 2013 Nov 25.
Published in final edited form as: Clin Cancer Res. 2012 Jul 2;18(17):10.1158/1078-0432.CCR-11-2776. doi: 10.1158/1078-0432.CCR-11-2776

Figure 2. Distinct phenotype of multipeptide-specific CTL (MP-CTL).

Figure 2

MP-CTL were generated from HLA-A2+ normal donors’ CD3+ T cells by repeated stimulation with irradiated APC pulsed with a cocktail of peptides (25 μg/ml; 6.25 μg/peptide) derived from XBP1 US, XBP1 SP, CD138, and CS1 antigen. One week after their fourth stimulation, the MP-CTL were evaluated for their phenotypic profile by flow cytometry. The MP-CTL (■) obtained from two different donors (Donor A, Donor B) showed a higher percentage of total CD8+ T cells as compared to the control unstimulated CD3+ T cells (□). The MP-CTL were further characterized as having higher frequencies of effector memory cells (CD45RO+CCR7−), higher activated (CD69+) cells and a corresponding lower percentage of naïve cells (CD45RO−CCR7+) within the CD3+CD8+ T cell subset.

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