Figure 5. Cytotoxic activity of MP-CTL against HLA-A2⁺MM cells including primary MM cells and cell lines.

Specific cytotoxic activity of the MP-CTL was analyzed one week after their fourth stimulation using a 4-hour calcein-release assay. MP-CTL (●) generated from T cells of Donor A (top) and Donor B (bottom) demonstrated effective lysis of both primary cells from two HLA-A2⁺ MM patients and HLA-A2⁺ U266 and McCAR MM cell lines. Control unstimulated T cells (▲) did not induce significant lysis of primary MM cells or cell lines.