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. 2013 Nov 25;33(6):e00082. doi: 10.1042/BSR20130090

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© 2013 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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(A) Processing of 30S rRNA. Total RNA was extracted from E. coli MG1693 (wt) and SK7622 (Δrnc) empty or containing pGEMt and pGEMt:rnc plasmids, and analysed in a 1.5% agarose gel. Bands were visualized by staining with ethidium bromide. The RNA species are indicated in the figure. This experiment was performed in quadruplicate. (B) PNPase regulation. Total soluble proteins were extracted from E. coli MG1693 (wt) and SK7622 (Δrnc) empty or containing pGEMt and pGEMt:rnc plasmids and 30 ng of protein were applied in a SDS/Page gel. A Western blot was performed using anti-PNPase and anti-RNase R antibodies. This experiment was performed in quadruplicate. PNPase levels were quantified and the values are indicated in the figure.