Figure 6.
The motogenic thrombospondin 1 (TSP-1)/ low-density lipoprotein receptor-related protein 1 (LRP1) mechanism inhibits Tcell receptor-induced T-cell proliferation. (a) The anti-CD47 antibody C1Km1 stimulates whereas B6H12 inhibits T-cell motility as determined by maximal depth of migration into a collagen gel. Migration was determined after 30 min. (b) 4N1K and C1Km1 inhibit proliferation. AF24 T cells were stimulated with antigen in the presence of various peptides and antibodies. The values show [3H]thymidine incorporation. (c) Anti-TSP-1 antibodies (Ab-3) stimulate anti-CD3-induced activation of AF24 as determined by [3H]thymidine incorporation. (a) Mean values of three independent experiments; (b, c) representative experiments of two or three independent experiments.